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. 2017 Feb 2;7:41608. doi: 10.1038/srep41608

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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C2C12 cells were transfected with negative control siRNA (si-NC) pool or Palmd siRNA pool (si-Palmd), then induced to differentiate 2d later. (A) The mRNA levels of Palmd 2d after differentiation, and Myogenin 12 h after differentiation, and MyHC, Mrf4 3d after differentiation, in two groups above. (B) The protein levels of Palmd, Myogenin and MyHC at the time points described in (A). (C) After transfection and differentiation for 3 days, MyHC was detected by immunofluorescence staining. Scale bar = 100 μm. (D) The differentiation index in (C) was calculated. 3 random microscopic fields were selected for each group. Data are presented as mean ± s.e.m., n = 3 per group. *p < 0.05, **p < 0.01, ***p < 0.001.