Figure 3.

Reduction in the expression of CD103 in MB from HIV-positive women. (A) The mean fluorescence intensity (MFI) of CD103 expression on CD3⁺CD8⁺ T cells. (B) The MFI of CD103 expression on CD3⁺CD4⁺ T cells. T cells were isolated from MB from HIV-positive and negative women and vaginal and endometrial samples from anonymous samples. (C) The MFI of CD103 expression on CD3⁺CD8⁺ T cells isolated from MB from HIV-negative and HIV-positive women stratified by absolute CD4 T cell number (cells/mm³) (D) Correlation between CD103 expression (MFI) on CD103⁺CD3⁺CD8⁺ T cells isolated from menstrual blood of HIV-positive women and the production of IFN-γ from CD4⁺CD3⁺ T cells isolated from menstrual blood of HIV-positive women specific for HIV. The IFN-γ production from CD4⁺ T cells from all HIV antigens demonstrating a positive response was added for each individual. (E) Correlation between CD103 expression (MFI) on CD103⁺CD3⁺CD8⁺ T cells isolated from menstrual blood and the percent of T-bet⁺ CD8⁺CD3⁺CD103⁺ T cells isolated from menstrual blood. (F) Sample gating strategy for T-bet from CD8⁺ T cells isolated from MB from an HIV-infected sample. Horizontal line represents median value. Statistically significant differences (P < 0.05) were obtained using the Wilcoxon Rank test for paired samples and the Mann Whitney test for unpaired samples. Although not noted in the graph, differences were also detected between MBC and tissues (VAG and ENDO). Correlations were determined using the Spearman correlation.