Figure 1.

Representative chromatogram of a depleted plasma sample prepared using affinity chromatography on an immobilized Frutalin column attached to an fast protein liquid chromatography system ÄKTApurifier 10 (GE Healthcare). Chromatography was performed at a constant flow of 1 ml/min and absorbance was monitored at 216 nm. The column was washed with a solution of 20 mM Tris–HCl, pH 7.4, 0.15 M NaCl. The non-retained peak (Unbound-PI fraction) was collected, and the bound material (retained peak, Bound-PII fraction) was eluted using d-galactose in a solution of 0.2 M Tris–HCl 20 mM, pH 7 4, 0.15 M NaCl.