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Dynamic range. After identification and absolute quantitation of the proteins using yeast alcohol dehydrogenase (red) as internal standard, alpha-1-antichymotrypsin (AACT_HUMAN) and alpha-1-antitrypsin (A1AT_HUMAN), represented by the blue and yellow spheres, respectively, were selected as housekeeping proteins used to normalize the data and perform label-free relative quantification of proteins present in the Unbound-PI and Bound-PII fractions, respectively.
