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Dependence of the changes in UV absorption intensity (plain lines) and shift in the absorption maximum from λ=261 to 268 nm (filled circles), which were determined for A) Dz15WS and B) Dz27WS at different pH values. A noncleavable version of the DNAzymes was used, in which adenosine at the cleavage site was replaced by its deoxy analogue. The following reaction conditions were applied: 50 mm sodium citrate, 25 °C; total concentration of DNAzymes 10 μm.
