Table 1.
The cleavage efficiency of trans‐acting DNAzymes Dz15WS_E (A) and Dz27WS_E (B) with oligonucleotide substrates Sub1, Sub2, Sub3, and Sub4, which contained additional ribonucleotides immediately adjacent to the cleavage site.
| Substrate | pH | Cleavage efficiency (A) [%] | Cleavage efficiency (B) [%] | ||||
|---|---|---|---|---|---|---|---|
| rU | rA | rG | rU | rA | rG | ||
| Sub1 27‐T rA G‐29 | 3.5 | – | 0.10 | – | – | 0.19 | – |
| 4.0 | – | 16.8 | – | – | 1.95 | – | |
| 4.5 | – | 62.4 | – | – | 15.44 | – | |
| Sub2 27‐rU rA G‐29 | 3.5 | 0.09 | 0.07 | – | 0.20 | 0.04 | – |
| 4.0 | 0.72 | 10.3 | – | 1.28 | 0.26 | – | |
| 4.5 | 1.52 | 50.1 | – | 0.0006 | 0.23 | – | |
| Sub3 27‐T rA rG‐29 | 3.5 | – | 0.02 | 0.05 | – | 0.04 | 0.06 |
| 4.0 | – | 0.37 | 0.05 | – | 0.02 | 0.03 | |
| 4.5 | – | 4.28 | 0.07 | – | 0.01 | 0.02 | |
| Sub4 27‐rU rA rG‐29 | 3.5 | 0.10 | 0.02 | 0.16 | 0.21 | 0.04 | 0.40 |
| 4.0 | 0.14 | 0.33 | 0.26 | 0.52 | 0.02 | 0.18 | |
| 4.5 | 0.17 | 2.01 | 0.22 | 0.11 | 0.01 | 0.14 | |