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. 2017 Feb 2;7:41806. doi: 10.1038/srep41806

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(A) Gene expression levels of BSEP in the HLCs as examined by real-time RT-PCR. The gene expression levels in PHH were taken as 1.0. Data represent mean ± SD (n = 3). (B) Immunofluorescence double staining of BSEP and ZO-1 in the different HLCs. White arrow indicates the co-localization of BSEP and ZO-1. (C) BEI of CLF-treated HLCs. Data represent mean ± SE (three independent differentiation experiments). Statistical significance was evaluated by one-way ANOVA in Tukey’s test. *P < 0.05. The scale bar represents 20 μm.