Figure 5. Relationship clarification of oxygen consumption, broth viscosity and biomass accumulation during fermentation of the MF1-IP strains.

OUR (oxygen uptake rate), k_La (mass-transfer coefficient) vs. oxygen volume content of the in-gas (A), viscosity vs. oxygen volume content of the in-gas (B), relationship between wet cell weight (WCW) and viscosity (C) of the MF1-IP and WT-IP strains in fed-batch fermentation. (D) chemostat culture of the MF1-IP strain with a dilution rate of D = 0.02 1/h. Culture and analysis methods were described in Materials and methods.