| E. coli |
| Top 10 |
F’[lacIq Tn10 (Tetr)] mcrA Φ80lacZ ΔM15 Δlac X74 deoR recA1
|
Invitrogen |
| P. pastoris |
| GS115 (Wild-type) |
his4 |
Invitrogen |
| GS115/pGLM1 |
GS115 PGAP:: pGLM1 (PGAP-GFP-Mig1 HIS4, Sh ble) |
This study |
| GS115/pGLM2 |
GS115 PGAP:: pGLM2 (PGAP-GFP-Mig2 HIS4, Sh ble) |
This study |
| Δmig1
|
GS115 mig1Δ::Sh ble his4
|
This study |
| Δmig2
|
GS115 mig2Δ::KAN his4
|
This study |
| Δnrg1
|
GS115 nrg1Δ::hph his4
|
This study |
| Δmig1Δmig2
|
GS115 mig1Δ::Sh ble mig2Δ::KAN his4
|
This study |
| Δmig1Δmig2Δnrg1
|
GS115 mig1Δ::Sh ble mig2Δ::KAN nrg1Δ::hph his4
|
This study |
| WT-Mit1 |
GS115 PGAP::pP6GM1 (PGAP-Mit1 HIS4, Bla) |
This study |
| WT-Prm1 |
GS115 PGAP::pPGP1 (PGAP-Prm1 HIS4, KAN) |
This study |
| Δmig1Δmig2-Mit1 |
GS115 mig1Δ::Sh ble mig2Δ::KAN PGAP::pP6GM1 (PGAP-Mit1 HIS4, Bla) |
This study |
| Δmig1Δmig2Δnrg1-Mit1 (MF1) |
GS115 mig1Δ::Sh ble mig2Δ::KAN nrg1Δ::hph PGAP::pP6GM1 (PGAP-Mit1 HIS4, Bla) |
This study |
| WT-GFP |
GS115 his4::pP-GFP (PAOX1-GFP HIS4) |
This study |
| Δmig1Δmig2-Mit1-GFP |
GS115 mig1Δ::Sh ble mig2Δ::KAN PGAP::pP6GM1 (PGAP-Mit1 Bla) his4::pP-GFP (PAOX1-GFP HIS4) |
This study |
| Δmig1Δmig2Δnrg1-Mit1-GFP (MF1-GFP) |
GS115 mig1Δ::Sh ble mig2Δ::KAN nrg1Δ::hph PGAP::pP6GM1 (PGAP-Mit1 Bla) his4::pP-GFP (PAOX1-GFP HIS4) |
This study |
| WT-IP |
GS115 his4::pPIC9K/IP (PAOX1-IP HIS4) |
This study |
| Δmig1Δmig2Δnrg1-Mit1-IP (MF1-IP) |
GS115 mig1Δ::Sh ble mig2Δ::KAN nrg1Δ::hph PGAP::pP6GM1 (PGAP-Mit1 Bla) his4::pPIC9K/IP (PAOX1-IP HIS4) |
This study |
