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. 2017 Feb 2;7:41777. doi: 10.1038/srep41777

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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At first the optimal route for the repopulation process was evaluated (A): When infused via the vascular system either islets were stuck inside the protein network of the vessels or were flushed through the organ. When infused via the pancreatic duct, islets leaked into peri-ductular parenchymal space. More islets were found in the pancreas repopulated via the pancreatic duct. Therefore further repopulation experiments were performed via the pancreatic duct (B): H/E staining confirmed the previous observations and showed many intact islets inside the decellularized space. TUNEL staining (red) revealed that islets are TUNEL negative and DAPI positive (blue) and therefore viable after the process.