FIG 2.

Quantifying RPA fluorescence in B-chip and plate reader assays. (A) A plot depicting the fluorescence intensity of RPAs to test isolated DNA from the following bacteria: A. baumannii (Ab), E. faecium (Ef), K. pneumoniae (Kp), P. aeruginosa (Pa), and S. aureus (Sa). We tested RPAs in the presence and absence of primers. (B) A plot depicting the fluorescence fold increase, which is a comparison of the RPA microchamber fluorescence in the presence of primers to the absence of primers for strains tested in panel A. (C) Comparison of the fluorescence fold increases between B-chip and the plate reader assay (n = 3).