FIG 5.

Determining the selectivity of primer-probe pairs in RPAs of samples containing DNA mixtures. (A) A plot of RPA fluorescence in microchambers containing bacterium-specific primers (green circles) or no primers (red circles) using samples containing a mixture of all 5 bacterial genomic DNAs at a concentration of 100 pg/μl. (B) A plot depicting the fold increase of fluorescence levels (ratio) between primer and no primer in samples consisting of mixtures of equal amounts of genomic DNA of all five organisms (n = 3). A. baumannii, Ab; E. faecium, Ef; K. pneumoniae, Kp; P. aeruginosa, Pa; S. aureus, Sa.