FIG 1.

Growth and uranium partitioning of Pelosinus sp. Strain UFO1. (A) Endpoint growth after 20 h of Pelosinus sp. strain UFO1 grown in the presence of increasing concentrations of uranium. (B) Strain UFO1 was grown in the presence of 25 μM (black bars) or 50 μM (gray bars) uranium. After growth, cells were harvested and total uranium was measured in the supernatant that could flow through a 0.22-μm filter (extracellular low molecular weight [LMW]), in the supernatant that was retained from flowing through a 0.22-μm filter (extracellular high molecular weight [HMW]), and in the pellet (cell associated). Values are reported as the percentages of total uranium added to the original culture. Error bars represent the standard deviations of values from biological triplicates.