FIG 2.

Purification of the uranium-binding complex from combined membrane and cytoplasmic fractions. The uranium-binding complex (UBC) was purified from the combined membrane and cytoplasmic fractions by two column chromatography steps, anion exchange (DEAE FF) (A) and size exclusion (Superose 6) (B). Fractions were then analyzed for protein (blue lines), uranium (red lines), and phosphorus (green line). The flowthrough fraction from column 1 was used for the second purification step and is denoted by a box in panel A. (C) Denaturing gel image for the purified UBC from fraction 6 of the Superose 6 column (arrow in panel B).