FIG 3.

Purification of the uranium-binding complex from the extracellular medium. The uranium-binding complex (UBC) was purified from extracellular medium by two column chromatography steps, anion exchange (DEAE FF) (A) and size exclusion (Superose 6) (B). Fractions were then analyzed for protein (blue lines), uranium (red lines), and phosphorus (green lines). The fractions from column 1 used for the second purification step are denoted by a box in panel A. (C) Denaturing gel image for the purified UBC from fraction 6 of the Superose 6 column (arrow in panel B).