FIG 5.
c-di-GMP represses the binding of FlrA to the promoter of the bpfA operon as determined by fluorescence polarization. (A) The binding of FlrA to FAM-labeled DNA covering box 2 of the promoter region was determined by fluorescence polarization. FAM-labeled DNA (15 nM) was incubated with FlrA at various concentrations. mA, mili-anisotropy value. (B) The binding of FlrA (450 nM) to this FAM-labeled DNA (FAM-DNA; 15 nM) was abolished by 25 μM c-di-GMP. The binding was outcompeted by unlabeled DNA with the same sequence (U-DNA) but not by unlabeled DNA with a mutation in box 2 (U-DNAM). Error bars indicate standard deviations from three samples.