FIG 5.

Multiple-sequence alignment of selected putative Nod and qNor enzymes around the quinol-binding site and the catalytic site of qNor. Representative environmental Nod enzymes were deduced from the gene sequences generated in this study (in bold). Only five of the longest environmental Nod sequences obtained in this study are included here (see Fig. S1 for extended alignments, including further environmental Nod enzymes). The conserved residues for quinol-binding and catalytic functioning in qNor are highlighted in red, whereas substitutions at these sites in putative Nod and putative Nor are shown in green. The accession numbers are the same as in Fig. 2. The alignment was generated with ClustalW in MEGA6.