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. Author manuscript; available in PMC: 2017 Feb 2.
Published in final edited form as: Free Radic Biol Med. 2013 Dec 1;68:22–34. doi: 10.1016/j.freeradbiomed.2013.11.015

Fig. 8.

Fig. 8

Effects of Zn on renal MT expression, Akt1 and Akt2 expression and phosphorylation, and HKII expression in MT-KO mice. Two-month-old male MT-KO (129S1 background) mice, 129S1 control mice, and FVB mice were given Zn (5 mg/kg) or physiological saline every other day for 1 month. (A) MT expression was detected by Western blot with antibody against MT1/2 isoforms. Akt expression and phosphorylation were detected by Western blot with antibodies against (B) Akt(1,2,3), (C) Akt1, and (D) Akt2. (E) GSK-3β expression and phosphorylation, as well as (F) HKII expression, was detected by Western blot assay. Data are presented as the mean ± SD (n = 6). *p < 0.05 vs corresponding controls (129S1 or FVB); #p < 0.05 vs 129S1/Zn group.