Table 1.
Genotypes of recombinant T. brucei cell lines generated in this study
| Targeting construct | T. brucei host strain | Genotype of recombinantsa |
|---|---|---|
| pG-ΔLII.EG | MITat 1.2 | Δep::(GFP::EP1ΔLII) NEO |
| pG-ΔLII.Luc | MITat 1.2 | Δep::LUCΔLII NEO |
| pL20-wt.EG | MITat 1.2 | RDNA::(GFP::EP1) BLE |
| pL20-ΔLII.EG | MITat 1.2 | RDNA::(GFP::EP1ΔLII) BLE |
| pL82-ΔLII.EG | MITat 1.2 13-90b | T7POL TETR NEO HYG RDNA::(GFP::EP1ΔLIITi) BLE |
| pTub-ΔLII.EG | MITat 1.2 | TUB::(GFP::EP1ΔLII) BLE |
| pL82.EG | MITat 1.2 13-90b | T7POL TETR NEO HYG RDNA::(GFP::EP1Ti) BLE |
| pL20.Luc | MITat 1.2 | RDNA::LUC BLE |
| pG-Δ164.EG | MITat 1.2 | Δep::(GFP::EP1Δ164) BLE |
| pL20-ΔUTR.EG | MITat 1.2 | RDNA::(GFP::EP1ΔUTR) BLE |
| pL20-ΔUTR.Luc | MITat 1.2 | RDNA::LUCΔUTR BLE |
| pG-ΔLII.EG | AnTat1.1 | Δep::(GFP::EP1ΔLII) NEO |
| pTR.EG | AnTat1.1, MITat 1.2 | RRNA::(GFP::EP1) HYG |
| pES221.EG | MITat 1.2 | VSG221::(GFP::EP1) NEO |
| pES221.E | MITat 1.2 | VSG221::EP1 NEO |
| pL82.E | AnTat1.1 90-13c | TETR T7POL NEO HYG RDNA::EP1Ti BLE |
a
Fusion genes are given in parentheses
b
Strain 13-90 carrying T7POL TETR NEO HYG was kindly provided by G.A.M. Cross, New York (Wirtz et al. 1999)
c
Miriam van den Bogaard and Mark Günzel (this lab)