Figure 1.

Effects of GroEL/S and DnaK/J overexpression on σ³²-dependent transcription. An exponential phase culture of strain 594 with a σ³²-dependent lacZ reporter and carrying either a plasmid able to overexpress GroEL/S (pGro7) or a plasmid able to overexpress DnaK/J (pKJE7) was grown at 30°C with and without 0.2% arabinose to induce GroEL/S or DnaK/J overexpression. A standard differential rate of synthesis plot is shown. The uninduced (control) strains gave identical results; therefore, for simplicity, we have included the data for only one control strain. This experiment and every other differential rate of synthesis experiment was performed at least three times with similar results.