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Changing the levels of GroEL/S increases the magnitude and duration of the heat shock response. An exponential phase culture of strain C600 or a derivative having the chromosomal groELS gene under control of the inducible Para promoter (CAG48176) was grown at 30°C and subjected to heat shock by increasing the temperature to 42°C. σ32 activity was measured by examining the rate of synthesis of HtpG.
