Fig. 10.

Hydrolysis and fermentation of washed industrial sugarcane straw using whole enzyme broth including fungal mycelia and other solids. Whole enzyme broth containing fungal mycelia and other solid residues was recovered from the final sample of the bioreactor cultivation of strain VTT-BR-C0022 on soybean hulls and sugarcane molasses (Fig. 9b). The whole enzyme broth was dosed in hydrolysis at 8, 12, and 16 mg/g. The reactions were carried out in two modes: with a 48-h pre-hydrolysis step at 45 °C followed by yeast addition and fermentation at 33 °C and pure simultaneous saccharification and fermentation carried out at 33 °C. The X-axis considers the time of both the hydrolysis and fermentation steps. The bottles were weighed periodically, and the loss of weight due to evolution of CO₂ was converted into values of cellulose to ethanol conversion (primary Y-axis) and ethanol titer (secondary Y-axis)