Fig. 3.

Production of enzymes by VTT-BR-C0019 in shake flasks on an inducing medium as compared to parental strain M44. T. reesei strains VTT-BR-C0019 and M44 were cultivated in shake flasks on an inducing medium comprising 4% milk whey, 4% Avicel and 1% yeast extract. a Culture supernatant samples from the last cultivation day (day 10) visualized on SDS-PAGE. Left lane Parental strain M44, Right lane: VTT-BR-C0019. Asterisks mark the clearly overexpressed proteins, likely corresponding to the main T. reesei xylanolytic enzymes. b Extracellular protein measured from cultivation samples of the parental strain M44 and VTT-BR-C0019. c Enzyme activity profile of the final cultivation day (day 10) samples. The bars represent the relative specific activities between the parental strain M44 and VTT-BR-C0019, while the numeric labels give the specific activities in units/milligram of protein