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. 2017 Feb 2;10:30. doi: 10.1186/s13068-017-0717-0

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Production of enzymes by VTT-BR-C0020 in shake flasks on an inducing medium as compared to the parental strain VTT-BR-C0019. T. reesei strains VTT-BR-C0020 and VTT-BR-C0019 were cultivated in shake flasks on an inducing medium (1% yeast extract, 4% milk whey, 4% Avicel). a Culture supernatant samples from the last cultivation day (day 10) visualized on a SDS-PAGE gel. Left lane Parental strain VTT-BR-C0019, right lane VTT-BR-C0020. Additional band corresponding to the heterologous beta-glucosidase is marked with an asterisk. b Extracellular protein measured from cultivation samples of the parental strain VTT-BR-C0019 and VTT-BR-C0020. c β-Glucosidase activity measured from cultivation samples of the parental strain VTT-BR-C0019 and VTT-BR-C0020