Table 2. Intramolecular cross-linked species identified by tandem MS.
| Exp. m/z | Δ, ppm | Peptide sequence | Cross-linked residues | Distances, Å |
|---|---|---|---|---|
| 816.143+ | 30 | Ffh 59-79 | Ffh K66-K76 (Tfh E66-A76)* | 15.7 |
| 583.335+ | 28 | Ffh 120-125, 272-289 | Ffh K122-K278 (Tfh L120-K275)* | 9.2 |
| 681.924+ | 23 | TftsY 87-101, 272-278 | TftsY K87-K274 | 19.7 |
| 581.594+ | 24 | TftsY 70-81, 272-278 | TftsY K71-K274 | 15.3 |
T. aquaticus Ffh and FtsY are annotated as Tfh and TftsY, respectively to distinguish from E. coli Ffh and FtsY. Exp. m/z is experimentally measured mass to charge ratio.
*
The cross-linked residues in E. coli complex were mapped back onto the corresponding T. aquaticus proteins as indicated in parentheses.