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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Biomaterials. 2016 Mar 19;92:1–12. doi: 10.1016/j.biomaterials.2016.03.024

Fig. 4.

Fig. 4

Collagen alignment and adaptive immune response-mediated in vivo calcification of leporine subpannicular BP implants. Representative images of 42 d BP implants on picrosirius red staining under polarized light (column 1) and von Kossa staining (Columns 2–4) (A). Scale bar = 100 μm. Collagen alignment of BP implants observed on picrosirius red staining and expressed as percent area of field of view (B). Adaptive immune response-mediated calcification of BP implants observed on von Kossa staining and expressed as percent area of field of view at sites of calcification (C). Results plotted as mean ± standard deviation (n = 6 per group). Groups not connected by same letter are significantly different (p < 0.05). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)