LRRK2 expression in BAC-transgenic G2019S rats (TG) and wild-type (WT). A. Western blot analysis of LRRK2 in striatum, cortex, hippocampus, and ventral midbrain. B, C. Expression above baseline was quantified in brain regions, where LRRK2 expression was readily detectable in WT. Expression was quantified relative to β-tubulin (LRRK2/β-tubulin) and normalized to the mean WT value. ***p <0.001 versus WT, Student’s t-test (n = 3/group; mean ± S.E.M.; LRRK2 expression analyzed at 8 months old).