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Search for paralogous TFs. By using the seed set of 1468 known human TF genes, we searched ScriptSure, a representation of the human transcriptome, using two methods: a high-stringency BLASTN search and an hmmpfam search for DNA-binding domains known to occur exclusively or primarily in TFs. The BLASTN search netted 3338 additional potential TFs, the domain search 2512. There were 494 genes that were found with both search methods; these 494 comprise the “found” set of human TF genes.
