Figure 5. Varying model parameters for integrase flipping and leaky expression.

1. As DNA flipping rates of intA ($k_{flipA}$) are decreased relative to $k_{flipB}$, the population of S _ab cells at ∆t = 0 h has a downward shift. Simulations are done with N = 3,000 trajectories/marker.
2. Increasing the leaky expression of intB ($k_{leakB}$) changes the maximum threshold of cells that correctly identify S _ab even at high ∆t. Leakiness is defined as a percentage of the induced integrase production rate ($k_{prod\ast }$).
3. The model was revised to more closely match the experimental data by constraining parameters for leaky expression and varying integrase flipping (N = 5,000). Mean squared error was calculated between the experimental data and the initial and revised models to find an optimized pair of $k_{flipA,B}$ values (Appendix Fig S13). The revised parameters are k _flipA = 0.2 h⁻¹, k _flipB = 0.3 h⁻¹, k _leakA = 1% of k _prodA(μm³ · h)⁻¹, and k _leakB = 2% of k _prodB(μm³ · h)⁻¹.