Table 1.

Screening Efficiency of Gateway Cloning

	Panel of selected ORFs
	A	B
ORF size range (bp)	500-3000	200-5500
Number of ORFs screened	111	192
4/4 positive colonies	72	108
3/4 positive colonies	11	28
2/4 positive colonies	06	15
1/4 positive colonies	06	08
0/4 positive colonies	16	33
Cloning efficiency (%)	86	83

The screening for recombinant master clones was performed by colony PCR on 4 colonies per each BP (gene) reaction. A positive colony represented the correct amplified gene size analyzed on agarose gel. DNA sequencing using plasmid-specific primers later confirmed the identity of the cloned genes.