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. 2016 Oct 8;79(1):115–122. doi: 10.1292/jvms.16-0329

Fig. 3.

Fig. 3.

Virus isolation from chickens inoculated with cell suspensions from MD chickens. (A) Day-old-chicks were inoculated with cell suspensions from the spleens of chickens with MD. Viruses were isolated by a primary CK cell culture at 2-3 wpi and passaged on CEFs 10 times. (B) Six and 12 plaques observed on CK cells from chickens 1 and 2 were purified and inoculated in CEFs, and the virus was passaged on CEFs 4 or 10 times. The picture indicates the detection of the GaHV-2, GaHV-3 and MeHV-1 genomes in DNA samples from 3 passaged viruses from CK cells of chicken 2. RB1B was used as a positive control for virulent GaHV-2, and CVI988 was used as a positive control for the attenuated GaHV-2 vaccine strain. SB-1 and Fc126 were used as positive controls for GaHV-3 and MeHV-1, respectively.