Table 1. Primers used to analyze the nucleotide sequences of the meq gene.
Primer | Type | Objective | Sequence | Size (bp) |
---|---|---|---|---|
M76S | FW | 1st round PCR | 5´-TTCCTAGGCAGGCGTCTCTTG-3´ | 1,132 |
M6S | RV | 5´-ATGGGGCATAGACGATGTGCT-3´ | ||
M10S | FW | Nested PCR | 5´-TGTCTCAGGAGCCAGAGCCGGGCGCT-3´ | 1,062 |
M10AS | RV | 5´-GGGGCATAGACGATGTGCTGCTGAG-3´ |