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. Author manuscript; available in PMC: 2017 Jun 19.
Published in final edited form as: Nat Immunol. 2016 Dec 19;18(2):152–160. doi: 10.1038/ni.3643

Figure 4. VGLL3 regulates genes associated with autoimmune diseases.

Figure 4

a, qRT-PCR of ITGAM, BAFF, and C3 upon RNAi of scrambled siRNA (Scr), VGLL3, UTX, ZFX, FEZ, FHL in primary human keratinocytes (n=3 independent experiments). b, qRT-PCR of VGLL3 in normal female (F) and male (M) skin (n=4 each). c, qRT-PCR of VGLL3 in primary human keratinocytes (n=4 each). d, immunohistochemistry of VGLL3 in healthy (Normal) and SLE patient (SLE) skin. Scale bar, 50 μm. e, log2(FC) and q-Value of the 10 female-biased immune transcripts upon VGLL3 RNAi in primary human keratinocytes from RNA-Seq experiments. f, literature-based network analysis of VGLL3-regulated autoimmune disease genes. g, log2(FC) levels of VGLL3 targets in skin of healthy (N) subjects and SCLE patients (SCLE) as well as upon VGLL3 RNAi. h, density plot of log2(FC) levels upon VGLL3 knockdown for SCLE and non-SCLE genes. P = 2.53 × 10−8. Mann-Whitney-Wilcoxon test. (a–c) Mean ± s.e.m, *, P<0.05, Student’s t-test.