Table 1.
In situ hybridization activity of CGRP, VEGF and OPN in the mandible and tibia.
| CGRP | VEGF | OPN | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| E12.5 | E14.5 | E17.5 | P1 | E12.5 | E14.5 | E17.5 | P1 | E12.5 | E14.5 | E17.5 | P1 | |
| Mandible | ||||||||||||
| Numerous mesenchymal cells at MC | + | + | - | - | + | + | ++ | ++ | - | - | - | - |
| Mesenchymal cells surrounding bone matrix | - | + | - | - | - | - | - | - | - | + | + | + |
| Mesenchymal cells surrounding blood vessels | - | + | - | - | - | - | - | - | - | - | - | - |
| Supra-surface | - | - | + | - | - | - | - | - | - | - | - | - |
| Basal region | - | + | - | - | - | - | - | - | - | - | - | - |
| Bone matrix | - | - | - | - | - | + | + | + | - | + | +++ | +++ |
| Nuclei | - | - | - | - | - | ++ | - | - | - | - | - | - |
| Cell membranes | - | - | - | - | - | ++ | - | - | - | - | - | - |
| Chondrocyte of MC | - | - | - | - | - | - | - | - | - | - | + | + |
| Tibia | ||||||||||||
| Numerous mesenchymal cells at HC | + | + | - | - | + | + | - | - | - | - | - | - |
| Hypertrophic zone | - | - | + | + | - | - | ++ | ++ | - | - | ++ | ++ |
| Bone matrix | - | - | - | - | - | - | + | + | - | - | +++ | +++ |
| Pre-hypertrophic zone | - | - | - | - | - | - | ++ | ++ | - | - | - | - |
MC, Meckel’s cartilage; HC, hypertrophic cartilage; -, not detected; +, scatter; ++, concentrate; +++, condense.