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. Author manuscript; available in PMC: 2017 Feb 3.
Published in final edited form as: Oncogene. 2016 Feb 15;35(36):4798–4806. doi: 10.1038/onc.2016.12

Figure 5.

Figure 5

The interaction between p53 and MDM4 decreases in resistant tumor cells grown at 32.5°C to restore cisplatin-induced p53 function. (A) A2780, 2780CP/Cl-16 and 2780CP/Cl-24 cells cultured at 37°C and 32.5°C, and cell lysates then prepared and subjected to Western blot using the indicated antibodies. (B) A2780, 2780CP/Cl-16 and 2780CP/Cl-24 cells cultured at 37 °C and 32.5 °C exposed to MG132 for 6 hours were collected, and cell lysates immunoprecipitated with the p53 antibody (FL-393) and the proteins immunoblotted with the indicated antibodies, as described in Figure 3. The immunoblots for MDM2 and MDM4 were developed using either a short or long exposure condition. Whole cell lysates were used as input. (C) Tumor cells grown at 32.5°C were exposed to vehicle or 1 μM (A2780) or 2 μM (2780CP/Cl-16 and 2780CP/Cl-24) cisplatin for 24 or 48 hours, and then collected for Western blot analysis using the indicated antibodies.