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. Author manuscript; available in PMC: 2018 Jan 26.
Published in final edited form as: Cell. 2017 Jan 26;168(3):377–389.e12. doi: 10.1016/j.cell.2016.12.033

Table 2.

LSD Dissociation and association rates at wild-type and mutant 5-HT2AR and 5-HT2BR.

Receptor BMAX ± SEM
fmol/mg protein		 Residence Time, min
(koff ± SEM) min−1 		kon ± SEM, M−1 min−1 Kd, nM
(pKd ± SEM)
5-HT2AR wild-type 2180 ± 350 221 (0.005 ± 0.001) 1.58×107 ± 4.06×106 0.33 (9.48 ± 0.11)
5-HT2aR L229AEL2 1650 ± 520 50 (0.020 ± 0.003) 3.34×107 ± 6.20×106 0.81 (9.22 ± 0.25)
5-HT2BR wild-type 3010 ± 614 46 (0.022 ± 0.004) 2.59×107 ± 3.04×106 0.91 (9.08 ± 0.09)
5-HT2bR L209AEL2 3628 ± 598 4 (0.236 ± 0.033) 4.20×107 ± 5.36×106 2.31 (8.63 ± 0.08)

Data were acquired by association and dissociation kinetic experiments conducted in parallel at 37°C using [3H]LSD (concentration range 0.2–5.0 nM). Estimates of koff, kon, and Kd were obtained from three independent experiments performed in duplicate. Residence time was calculated as 1/koff.