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Overview of the RNAi screening procedure. (A) Overnight cultures of 96 bacterial clones are inoculated in sixteen 24-wells plates (six clones per plate and four wells per clone). After overnight induction of the dsRNA synthesis, three to 10 worms synchronized at the L1-stage (N2 or lin-35 strain) were deposited into the wells. (B) RNAi experiments were performed at 20°C. We observed a wide range of phenotypes across development.
