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. Author manuscript; available in PMC: 2017 Sep 28.
Published in final edited form as: J Control Release. 2016 Jul 30;238:176–185. doi: 10.1016/j.jconrel.2016.07.051

Figure 7.

Figure 7

Confocal micrographs of uptake of nanoparticles of different shapes and surface chemistries by Caco-2/HT-29 cells. Representative confocal microscopy images showing uptake of A) Spheres; B) Rods; C) Discs; D) Biotin conjugated Spheres; E) Biotin-conjugated Rods and F) Biotin-conjugated Discs by Caco-2/HT-29 cells at the end of 5 h of study. Green fluorescence represents particles while blue color represents cell nuclei (DAPI staining). Scale bar = 10 µm. It is to be noted that upon stretching 200 nm spheres into rods and discs, the fluorescence of the stretched particles diminishes by approximately 1.5 and 3 fold respectively compared to spheres. Therefore, despite attenuated fluorescence, rods demonstrate higher uptake than spheres in Caco-2/HT-29 cells.