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. 2017 Feb 2;12(2):e0170197. doi: 10.1371/journal.pone.0170197

Fig 4. Apigenin inhibits CK2 activity and improves PP1 expression, in vivo.

Fig 4

(A) Western blot analysis of CK2α protein expression in Control (CTRL), TB and TB-API splenocytes. (B) Counts per minute (C.P.M.) of CK2 activity in protein lysates from splenocytes from CTRL, TB and TB-API mice as assayed by an in vitro CK2 kinase assay (C) Western blot analysis of PP1 protein expression in CTRL, TB and TB-API splenocytes. (D) and (E) representative quantification of normalized densitometric ratios of western blots data of CK2 and PP1, respectively. To control for equal protein loading, the blots were reprobed with an antibody specific to β-actin. Graphs represented are the mean ± S.E.M. of CTRL (n = 3), TB (n = 3) and TB-API (n = 3) mice of three independent experiments. *p<0.05; (by two-tailed Student’s t test).