Table 1. Primers used for generating polymorphic ISSR markers in Pilocarpus microphyllus genotypes, showing the respective levels of polymorphic loci.
Primer [repetitions]a | Polymorphic loci |
---|---|
844 [(CT)8 RC] | 14 |
CHRIS [(CA)7 YG] | 13 |
DAT [(GA)7 RG] | 9 |
ISSR-4 [(AC)8 YT] | 9 |
ISSR-6 [(AG)8 YT] | 8 |
843 [(CT)8 RA] | 9 |
JOHN [(AG)7 YC] | 5 |
MANNY [(CAC)4 RC] | 10 |
MAO [(CTC)4 RC] | 12 |
OMAR [(GAG)4 RC] | 14 |
TERRY [(GTG)4 RC] | 8 |
a Degenerate bases used: Y (C or T), R (A or G).