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. 2017 Jan 5;5:e2859. doi: 10.7717/peerj.2859

Figure 2. miR-21 effect of on CSC proliferation.

Figure 2

(A) Cultured CSCs were treated with miR-21 mimics or its negative control scramble for 24 h, 48 h or 72 h respectively. Cell proliferation and viability were detected with CCK-8 assay. miR-21 mimics remarkably increased proliferation of c-kit+ CSC at the three time points (with P values < 0.05) and 48 h was the peak point of cell viability. n = 3. (B) c-kit+ CSCs were double stained by c-kit (green) and DAPI (blue), and observed under a fluorescence microscope (Olympus). Bar = 50 µm. DAPI = propidium iodide. (C) The statistics of EdU positive CSCs from immunofluorescence in (B). n = 6 in each group. (D) miR-21 mimics’s in-fluences on BrdU expression, which was detected with immune blotting. miR-21 mimics dramatically increased the expression of BrdU compared with Control or Scramble group. n = 3. *, P < 0.05.