Figure 3. miR-21 effect of on PTEN expression in CSCs.

Cultured CSCs were treated with miR-21 mimics or its negative control scramble for 48 h, then cells were harvested and subjected to RT-PCR or Western blot. PTEN mRNA of Control, scramble treated or miR-21 mimics treated CSCs showed no significant difference (A), but PTEN protein dramatically decreased after miR-21 mimics treatment (B). a, P < 0.05 compared with Control; b, P < 0.05 compared with Scramble. n = 3 in each group.