Fig 2. HBV/HBx requires extracellular Ca²⁺ influx to elevate [Ca²⁺]_c.

(A) Primary rat hepatocytes were loaded with 5 μM Fura-4F and stimulated with 100 μM ATP in Ca²⁺-free buffer (0 mM Ca²⁺) or in Ca²⁺-containing buffer (2 mM Ca²⁺). (B and C) Control and HBx-expressing primary rat hepatocytes were loaded with 5 μM Fura-4F and stimulated with 100 μM ATP in Ca²⁺-free buffer (0 mM Ca²⁺). Calculations were performed as described for Fig 1. The data represent the means ± SE and are taken from at least three experiments from different hepatocyte preparations. **P < 0.01