Fig 4. SOCE is required for HBV replication.

(A and B) Primary rat hepatocytes were loaded with 5 μM Fura-4F and treated with 2 μM TG in Ca²⁺-free buffer, followed by treatment with DMSO or the SOCE inhibitor 50 μM APB (A) or 100 nM La³⁺ (B) before the addition of 1 mM CaCl₂ (Ca²⁺). Calculations were performed as described for Fig 3. The data represent the means ± SE and are taken from at least three experiments from different hepatocyte preparations. (C-E) Primary rat hepatocytes were infected with a recombinant adenovirus expressing HBV (AdHBV) and treated with the SOCE inhibitors for 48 hrs or infected with a recombinant adenovirus expressing an HBx-deficient HBV (AdHBV*7). Levels of HBV replication (Southern blot) (C), HBV core protein expression (western blot) (D), and HBV mRNAs (northern blot) (E) were assessed.