Fig 4. Hyphal growth assay in presence of the 3 NSAIDs in the VDA collection.

Hyphal growth was imaged after 24h of growth at 37°C in low glucose conditions using a strain expressing GFP-YPT72. Cells were challenged with 0.5% DMSO (A), 50 μM diflunisal (B), 50 μM tolfenamic acid (C), and 50 μM mefenamic acid (D). Images were acquired using a BioTek Synergy imaging reader at a 60X magnification for bright field imaging (left panels) and fluorescence (eGFP excitation filter of 485/20; right panels). The error bars represent 20 microns.