Fig 3. Dt81Hepa1-6 cells are specific to the liver.
A suspension of 1M Dt81Hepa1-6 cells was injected subcutaneously (SC) in the left flank of C57BL/6 mice and animals were sacrificed after 28 days. (A) AFP mRNA relative expression in control and SC injected C57BL/6 mice. (B) Whole liver photographs at time of sacrifice. HPS-stained slices representative microphotographs of (C) lungs and spleen after SC injection and (D) Lungs and spleen after IS injection. Representative microphotographs were obtained at 100x magnification. (*P<0.05).