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. 2017 Feb 2;12(2):e0171339. doi: 10.1371/journal.pone.0171339

Fig 2. Phylogenetic analysis of plasmid-encoded relaxase homologs.

Fig 2

Plasmid pHS091147 (in bold) and twelve other protein sequences were aligned, and the tree was generated with MEGA5 using the maximum-likelihood method. Solid black arrows point to the prototype plasmids for the MOBF12 and MOBF11 subfamilies. Other relaxase sequences of plasmids pKP048 (GenBank accession number FJ628167), pHS082416 (KF724507), pKHS2 (CP003224), pKP09085 (KF719970), pKPN4 (CP000649), pKPX-2 (AP012056), pK1HV (HF545434), F (AP001918), pSLT (AE006471), pED208 (AF411480), pET45 (CU468132) and R388 (BR000038) were obtained from GenBank.