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(B) western blot for the cleaved TLR9 product in rat PAEC cells exposed for 24 h with either CpG DNA (20 ug/ml) or Hb (25 uM heme). (B) western blot for the cleaved TLR9 product in rat PAEC exposed to Hb, Hb bound to Hp (Hb-Hp), or co-treated with Hb plus hpx (Hb+Hpx). * p<0.04 vs ctrl. Hb- hemoglobin Hp-haptoglobin, Hpx- hemopexin. Experiments were completed with a minimum of 2 individual cell preparations per group on four separate days (n = 8).
