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. 2017 Feb 2;12(2):e0171219. doi: 10.1371/journal.pone.0171219

Fig 3. Lipid peroxidation, cell death and nucleic acid accumulation.

Fig 3

(A) Photomicrographs that show areas of lipid perioxidation in rPAEC exposed to Hb (25 uM heme) in the presence or absence of haptoglobin or hemopexin. (B) Image J quantification of lipid peroxidation. (C) LDH levels in culture supernatant in rPAEC expose to Hb. (D) Nucleic acid concentrations in culture supernatant exposed to Hb. (E) HEK-blue TLR9 reporter cell activity in cells treated with Hb in the presence or absence of DNASE. *p<0.04 vs. ctrl. Green lipid peroxidation- (White arrows). Hb- hemoglobin Hp-haptoglobin, Hpx- hemopexin.