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. 2017 Feb 2;12(2):e0171313. doi: 10.1371/journal.pone.0171313

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© 2017 Pogrebnyakov et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — The library was constructed using the RBS Calculator and fused to the P_pfl promoter of G. thermoglucosidasius C56-YS93 and sfGFP. (A) Sequences of RBS (grey background) and their strengths (standard deviations in parentheses) as measured by sfGFP fluorescence. (B) RBS activity compared to the predicted translation initiation rate. RBS’ on the horizontal axis of the graph appear in the same order as in the table.